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alp substrate 287 kit  (Bio-Rad)


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    Bio-Rad alp substrate 287 kit
    Alp Substrate 287 Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alp substrate 287 kit/product/Bio-Rad
    Average 93 stars, based on 176 article reviews
    alp substrate 287 kit - by Bioz Stars, 2026-02
    93/100 stars

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    Maternal exercise alters growth plate morphometry and bone neovascularization. (A) Fluorescent staining for collagen type 10 alpha 1 chain (Col10a1) in the proximal hypertrophic zone (HZ) of embryonic day 17.5 (E17.5) humeri. Scale bar = 500 μm. (B) HZ length. (C) Fluorescent staining for alkaline <t>phosphatase</t> <t>(ALP)</t> activity in E17.5 humeri. Scale bar = 250 μm. (D) Primary ossification center (POC) ALP activity and (E) bone collar (BC) ALP activity. (F) Fluorescent staining for blood vessels (endomucin) in E17.5 humeri. Scale bar = 250 μm. (G) Medullary vessel area, (H) Medullary vessel density, (I) BC vessel area, (J) BC vessel density, (K) metaphyseal vessel area, and (L) metaphyseal vessel density. Fetuses from the same litter are marked by the same color datapoint. * p < 0.05 using a Student's t ‐test.
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    Differentiation features upon DM treatment. (A) Alkaline phosphatase <t>(ALP)</t> activity visualized at day 7 by <t>histological</t> <t>staining.</t> Scale bar: 100 µm. (B) ALP enzymatic assay measured at day 7 and normalized to Crystal violet signal for cell content. (C). Gene expression of SFTP‐C after 7 days in SM or DM analyzed by RT‐qPCR (* p < 0.01, *** p < 0.0001). (D) Immunostaining showing SFTP‐C signal (red) with DAPI (blue) used as nuclear counterstain in A549 treated for 7 and 10 days. Scale bar: 50 µm.
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    Image Search Results


    Maternal exercise alters growth plate morphometry and bone neovascularization. (A) Fluorescent staining for collagen type 10 alpha 1 chain (Col10a1) in the proximal hypertrophic zone (HZ) of embryonic day 17.5 (E17.5) humeri. Scale bar = 500 μm. (B) HZ length. (C) Fluorescent staining for alkaline phosphatase (ALP) activity in E17.5 humeri. Scale bar = 250 μm. (D) Primary ossification center (POC) ALP activity and (E) bone collar (BC) ALP activity. (F) Fluorescent staining for blood vessels (endomucin) in E17.5 humeri. Scale bar = 250 μm. (G) Medullary vessel area, (H) Medullary vessel density, (I) BC vessel area, (J) BC vessel density, (K) metaphyseal vessel area, and (L) metaphyseal vessel density. Fetuses from the same litter are marked by the same color datapoint. * p < 0.05 using a Student's t ‐test.

    Journal: The FASEB Journal

    Article Title: Maternal Exercise Rescues Fetal Akinesia‐Impaired Joint and Bone Development

    doi: 10.1096/fj.202503192R

    Figure Lengend Snippet: Maternal exercise alters growth plate morphometry and bone neovascularization. (A) Fluorescent staining for collagen type 10 alpha 1 chain (Col10a1) in the proximal hypertrophic zone (HZ) of embryonic day 17.5 (E17.5) humeri. Scale bar = 500 μm. (B) HZ length. (C) Fluorescent staining for alkaline phosphatase (ALP) activity in E17.5 humeri. Scale bar = 250 μm. (D) Primary ossification center (POC) ALP activity and (E) bone collar (BC) ALP activity. (F) Fluorescent staining for blood vessels (endomucin) in E17.5 humeri. Scale bar = 250 μm. (G) Medullary vessel area, (H) Medullary vessel density, (I) BC vessel area, (J) BC vessel density, (K) metaphyseal vessel area, and (L) metaphyseal vessel density. Fetuses from the same litter are marked by the same color datapoint. * p < 0.05 using a Student's t ‐test.

    Article Snippet: Alkaline phosphatase (ALP) staining was conducted using the Vector blue Alkaline Phosphatase (ALP) substrate kit (SK‐5300, Vector Laboratories, Newark, CA, USA) according to the manufacturer's instructions.

    Techniques: Staining, Activity Assay

    Differentiation features upon DM treatment. (A) Alkaline phosphatase (ALP) activity visualized at day 7 by histological staining. Scale bar: 100 µm. (B) ALP enzymatic assay measured at day 7 and normalized to Crystal violet signal for cell content. (C). Gene expression of SFTP‐C after 7 days in SM or DM analyzed by RT‐qPCR (* p < 0.01, *** p < 0.0001). (D) Immunostaining showing SFTP‐C signal (red) with DAPI (blue) used as nuclear counterstain in A549 treated for 7 and 10 days. Scale bar: 50 µm.

    Journal: Advanced Biology

    Article Title: Differentiation Treatment Applied to Lung Cancer Model Reduces Pathogenic Traits in Vitro

    doi: 10.1002/adbi.202500371

    Figure Lengend Snippet: Differentiation features upon DM treatment. (A) Alkaline phosphatase (ALP) activity visualized at day 7 by histological staining. Scale bar: 100 µm. (B) ALP enzymatic assay measured at day 7 and normalized to Crystal violet signal for cell content. (C). Gene expression of SFTP‐C after 7 days in SM or DM analyzed by RT‐qPCR (* p < 0.01, *** p < 0.0001). (D) Immunostaining showing SFTP‐C signal (red) with DAPI (blue) used as nuclear counterstain in A549 treated for 7 and 10 days. Scale bar: 50 µm.

    Article Snippet: ALP staining was performed with the SK‐5400 detection kit (Vector Laboratories) according to the manufacturer's instructions, and imaged on a Leica DMIL microscope.

    Techniques: Activity Assay, Staining, Enzymatic Assay, Gene Expression, Quantitative RT-PCR, Immunostaining

    Spheroids formation in 3D cultures of A549 in SM or DM. (A) A549 spheroids at day 7 (top) and 14 (bottom). Scale bar: 500 µm. (B) Phalloidin (red) staining of day 7 spheroids in SM or DM medium, with DAPI nuclear counterstain. Scale bar: 500 µm. (C) Spheroid diameter measured over 14 days. (D) RT‐qPCR expression analysis of stem cell markers SOX2, CD44, and NANOG at day 7. (E) ALP staining (dark blue) in spheroids processed by Cytospin. Scale bar: 100 µm. (* p < 0.01, ** p < 0.001, *** p < 0.0001).

    Journal: Advanced Biology

    Article Title: Differentiation Treatment Applied to Lung Cancer Model Reduces Pathogenic Traits in Vitro

    doi: 10.1002/adbi.202500371

    Figure Lengend Snippet: Spheroids formation in 3D cultures of A549 in SM or DM. (A) A549 spheroids at day 7 (top) and 14 (bottom). Scale bar: 500 µm. (B) Phalloidin (red) staining of day 7 spheroids in SM or DM medium, with DAPI nuclear counterstain. Scale bar: 500 µm. (C) Spheroid diameter measured over 14 days. (D) RT‐qPCR expression analysis of stem cell markers SOX2, CD44, and NANOG at day 7. (E) ALP staining (dark blue) in spheroids processed by Cytospin. Scale bar: 100 µm. (* p < 0.01, ** p < 0.001, *** p < 0.0001).

    Article Snippet: ALP staining was performed with the SK‐5400 detection kit (Vector Laboratories) according to the manufacturer's instructions, and imaged on a Leica DMIL microscope.

    Techniques: Staining, Quantitative RT-PCR, Expressing